Rhizosphere community selection reveals bacteria associated with reduced root disease

Abstract Background Microbes benefit plants by increasing nutrient availability, producing plant growth hormones, and protecting against pathogens. However, it is largely unknown how change root microbial communities. Results In this study, we used a multi-cycle selection system infection the soilborne fungal pathogen Rhizoctonia solani AG8 (hereafter AG8) to examine impact rhizosphere bacterial community recruit beneficial microorganisms suppress pathogens promote growth. Successive plantings dramatically enhanced disease suppression on susceptible wheat cultivars in greenhouse. Accordingly, analysis of soil using deep sequencing 16S rRNA genes revealed distinct profiles assembled over successive plantings. Moreover, cluster communities formed from AG8-infected was those without infection. Interestingly, with lowest gradually separated worst planting cycles. monocultures application increased abundance some genera which have potential antagonistic activities, such as Chitinophaga , Pseudomonas Chryseobacterium Flavobacterium group growth-promoting (PGP) nitrogen-fixing microbes, including Pedobacter Variovorax Rhizobium . Furthermore, 47 bacteria isolates belong 35 species were isolated. Among them, eleven five exhibited activities oryzae vitro respectively. Notably, Janthinobacterium displayed broad antagonism Pythium ultimum AG8, R. suppressive activity soil. Conclusions Our results demonstrated that can shape specifically accumulate microbes. findings suggest may offer for addressing agronomic concerns associated diseases crop productivity.